Not known Factual Statements About thr77
Not known Factual Statements About thr77
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide normally contains the epitope recognized via the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs get more info which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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